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My puppy has a UTI. How long off antibiotics for a urine culture?

Updated On September 23rd, 2025

Pet's info: Dog | Newfoundland | Female | unspayed | 3 months and 25 days old | 50 lbs

How long should a 4 month puppy be off of an antibiotic prior to a urine culture? We are visiting a specialist for an UTI after three courses of antibiotics.

4 Answers

Most Helpful Answer

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Answered By Dr. Heidi DVM, CVA, CCRT, CVTP, CVSMT, CVCH

Veterinarian, Certified Veterinary Acupuncturist, Certified Canine Rehabilitation Practitioner

Published on February 23rd, 2018

In general, a dog should be off antibiotics for 10-14 days prior to a urine culture, but sometimes we will culture a dog that has not been off antibiotics that long if we suspect that there is still an infection. I would certainly discuss that with the specialist and get their recommendation. I'm so sorry that you are dealing with a challenging problem in such a young puppy, but I hope the specialist is able to help Jett. Good luck! I hope that was helpful and answered your question. Thanks for using Petco Pet Education Center, formerly Petcoach.

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    Answered By Angel Alvarado, LVT

    Licensed Veterinary Technician

    Published on November 9th, 2017

    You should begin to see some improvement in symptoms after a few days IF the bacteria responsible is sensitive to the antibiotic. Ideally, a sterile urine sample should be collected and submitted for urinalysis and a urine culture/sensitivity panel. This second test should be able to identify the organism responsible as well as the most effective antibiotic to use in treatment.

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    Answered By Dr. Elizabeth

    Veterinarian

    Published on November 19th, 2019

    It is generally expected that the antibiotics will kick in and the clinical signs should subside by day 5. I would be concerned there is antibiotic resistance, a bladder stone or bladder cancer causing the continued symptoms but it could also just be a slow response. I recommend to call you vet and decide what to do next. Best of luck and please feel free to post any additional questions.

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    Answered By Andrea M. Brodie, DVM

    Veterinarian

    Published on August 19th, 2018

    It can take up to six weeks of taking antibiotics to combat a UTI. You will need to be patient. 4 days of treatment is a very short time and you will likely not see any results for another few days. Make sure he drinks a lot, and go for walkies and the more often he urinates, the better, as he is getting rid of bacteria in his bladder.

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